Agnieszka Bak
Reinheim
Summary
Dedicated Research Associate nearing completion of a PhD with a strong background in molecular genetics, animal biotechnology, and in vitro assays. Skilled in DNA/RNA isolation, cell culture, primary cell isolation, and Seahorse metabolic flux analysis for studying oxidative stress, cell death pathways, and metabolic bioenergetics. Proficient in PCR, qPCR, FISH (Fluorescence In Situ Hybridization), flow cytometry, and advanced data interpretation, driving innovative outcomes in fast-paced research settings. Excels in multitasking, collaboration, and delivering high-quality results to support advanced biomedical research.
Overview
14
14
years of professional experience
1
1
Certification
Work History
Research Associate
Chair of Livestock Biotechnology, TU München
04.2021 - 06.2024
- Investigated AK1P1's role in oxidative stress and cell death pathways in a pig model
- Utilized advanced in vitro assays, including Seahorse metabolic flux analysis, to measure cellular bioenergetics (mitochondrial respiration, glycolysis) and viability
- Isolated and cultured primary cells, optimizing protocols for robust experimental reproducibility and data integrity
- Analyzed data using GraphPad and statistical models, delivering high-quality presentations and reports
- Collaborated with researchers and scientists to design and execute experiments.
- Developed new protocols and improved existing laboratory processes.
Scientist
Chair of Livestock Biotechnology, TU München
12.2019 - 03.2021
- Led 3 key projects in xenotransplantation, employing DNA/RNA isolation and primary cell culture techniques to assess tissue compatibility
- Collaborated with cross-functional teams to interpret results and refine experimental designs
- Mentored junior scientists, elevating overall expertise within team.
Intern
Chair of Livestock Biotechnology, TU München
02.2018 - 01.2019
- Analyzed driver genes for osteosarcoma in TP53-targeted pigs, using PCR and cell culture methods
- Supported senior researchers with data collection, resource planning, and preliminary reporting.
Scientist
Dept. of Genomics & Animal Molecular Biology, Natl. Research Inst. of Animal Production, Balice
01.2016 - 10.2017
- Participated in cytomolecular analyses of HSP genes in horses (Equus caballus), performing FISH and microscopy
- Contributed to multi-phase research initiatives, documenting results and troubleshooting lab protocols.
Laboratory Assistant
Dept. of Genomics & Animal Molecular Biology, Natl. Research Inst. of Animal Production, Balice
10.2010 - 12.2015
- Performed DNA & RNA isolation, BAC culture media prep, cytomolecular diagnostics, and PCR for gene sequencing
- Conducted practical classes for students on experimental design and in vitro methodologies
- Maintained safe, tidy and organized laboratory environment for accurate test results.
Education
PhD - Biotechnology
TU München
Freising01.2026
MSc - Biotechnology
University of Silesia
Katowice12.2009
MSc - Chemistry
Jan Dlugosz University
Czestochowa07.2007
BSc - Biotechnology
Jan Dlugosz University
Czestochowa06.2005
Skills
- Cell Culture & Primary Cell Isolation: mammalian cell/tissue culture techniques (2D- and 3D-cultures)
- Project Management & Collaboration: Interdisciplinary teamwork, mentoring, effective communication
- Molecular Biology Techniques: DNA/RNA isolation, qPCR, PCR, ddPCR
- Assay Design & Optimization: Seahorse metabolic flux, oxidative stress, apoptosis/necrosis pathways
Certification
- Industrial Pharma Management
- BioTech Quality Manager
- GMP Compliance for Diclofenac Ointment
LANGUAGES
English: Full Professional Proficiency
German: Intermediate Proficiency
Polish: Native
Work Availability
monday
tuesday
wednesday
thursday
friday
saturday
sunday
morning
afternoon
evening
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Affiliations
- TU München
Work Preference
Work Type
Full TimeWork Location
On-SiteHybridImportant To Me
Career advancementCompany CulturePersonal development programsTeam Building / Company RetreatsWork-life balanceTimeline
Research Associate
Chair of Livestock Biotechnology, TU München
04.2021 - 06.2024
Scientist
Chair of Livestock Biotechnology, TU München
12.2019 - 03.2021
Intern
Chair of Livestock Biotechnology, TU München
02.2018 - 01.2019
Scientist
Dept. of Genomics & Animal Molecular Biology, Natl. Research Inst. of Animal Production, Balice
01.2016 - 10.2017
Laboratory Assistant
Dept. of Genomics & Animal Molecular Biology, Natl. Research Inst. of Animal Production, Balice
10.2010 - 12.2015
PhD - Biotechnology
TU München
MSc - Biotechnology
University of Silesia
MSc - Chemistry
Jan Dlugosz University
BSc - Biotechnology
Jan Dlugosz University
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