Charles Christian Mugisha
Berlin,Land Berlin
Summary
Accomplished and reliable Biological Laboratory Technician with over five years of experience in research, diagnostics, and industrial laboratory operations. Skilled in managing workflows, coordinating teams, and ensuring compliance with quality and safety standards. Driven by a strong commitment to scientific excellence and continuous learning, with a proactive approach to improving laboratory efficiency and supporting collaborative success.
Overview
6
6
years of professional experience
4
4
years of post-secondary education
1
1
Certification
Work history
Laboratory manager
AG Hegazy/ Charité-DRFZ
Berlin, Land Berlin
01.2025 - Current
- Assisted in procurement and maintenance of laboratory materials and equipment.
- Supported preparation of chemical solutions.
- Contributed to establishment and optimisation of new methods and protocols.
- Facilitated set up of new FACS panels and method evaluation.
- Conducted cell culture cultivation of primary cell lines, organoids, and cryopreservation.
- Performed microscopy tasks.
- Executed cloning and transfection using calcium phosphate immunoprecipitation.
- Conducted serial extraction, electrophoresis, ELISA, PCR, and protein purification.
- Executed SDS-PAGE and Western blot analyses.
- Isolated nuclei from FFPE blocks for sequencing.
Laboratory technician
AG Hemmer/ MRI-TUM
Munich, Bavaria
08.2021 - 12.2023
- Procured and maintained laboratory materials, reagents, and equipment.
- Prepared standard and customized laboratory solutions.
- Developed, optimized, and validated new experimental methods and protocols.
- Managed biobank inventory, sample documentation, and data records.
- Performed FACS-based diagnostics and evaluated analytical methodologies.
- Conducted cell culture work, including cultivation of HEK cells, PBMC isolation, and cryopreservation.
- Operated various microscopy techniques for cellular and molecular analysis.
- Executed molecular cloning, transfection (calcium phosphate), and immunoprecipitation experiments.
- Conducted serial extractions, electrophoresis, ELISA, PCR, and protein purification assays.
- Managed SDS-PAGE and Western blot projects for protein detection and quantification.
- Contributed to research project on DNA demethylation in multiple sclerosis.
Lab manager
AG Heine/UKSH
Kiel, Schleswig-Holstein
01.2020 - 08.2021
- Oversaw procurement and maintenance of laboratory equipment, instruments, and consumables.
- Supervised preparation of laboratory solutions and reagents.
- Developed, optimized, and implemented new experimental methods and protocols.
- Managed Biobank operations, including sample documentation, data tracking, and compliance with quality standards.
- Determined protein lysate concentrations using Biuret assay.
- Performed PBMC isolation and cryopreservation according to standardized procedures.
- Conducted transfection experiments (calcium phosphate method) and immunoprecipitation assays.
- Operated and analyzed samples using flow cytometry (FACS).
- Executed PCR and RT‑PCR for nucleic acid amplification and gene expression analysis.
- Isolated high‑quality DNA and RNA for molecular studies.
- Performed ELISA for quantitative protein detection.
- Conducted Western blot analyses for protein identification and validation.
Education
Certified Biological Technician -
Biological Technician Assistent
Hamburg 09.2017 - 08.2019
Medical Technician assistant for functional Diagnostic
Göttingen, Lower Saxony 07.2013 - 08.2015
Skills
Skills
- Flow Cytometry (FACS)
- Hematology & Hemostasis
- Immunoassays
- Laboratory Best Practices & Quality Standards
- Human Anatomy & Physiology
- Microscopy Techniques
- Microsoft Office Suite (Excel, Word, PowerPoint)
Soft Skills
- Strong Team Collaboration
- Excellent Communication Skills
- Analytical and Problem-Solving Mindset
- High Sense of Responsibility and Reliability
Languages
English
Fluent
German
Fluent
French
Fluent
Kinyarwanda
Native
Swahili
Fluent
Spanish
Intermediate
Arabic
Elementary
Affiliations
- Enjoy reading scientific and non-fiction literature, exploring new cultures through travel, engaging in creative writing, and maintaining an active lifestyle through mountain hiking.
Certification
FELASA B
References
References available upon request.
Timeline
Laboratory manager
AG Hegazy/ Charité-DRFZ
01.2025 - Current
Laboratory technician
AG Hemmer/ MRI-TUM
08.2021 - 12.2023
Lab manager
AG Heine/UKSH
01.2020 - 08.2021
Certified Biological Technician -
Biological Technician Assistent
09.2017 - 08.2019
Medical Technician assistant for functional Diagnostic
07.2013 - 08.2015