Dr. Anusree Lakshmi Sivadas

Curious and hardworking Postdoctoral researcher trying to unravel the mystery behind cancer progression and metastasis with a strong backing of molecular biology. I studied the molecular mechanisms underlying nuclear RNA- surveillance during my PhD and transitioned into cancer research for my first Postdoctoral position. Currently, using my expertise in molecular biology to understand the intricacies of Pancreatic ductal adenocarcinoma.

• The zinc-finger protein Red1 orchestrates MTREC submodules and binds the Mtl1 helicase arch domain, Nature Communications, 12, 3456, 2021

• Mechanistic insights into RNA surveillance by the canonical poly(A) polymerase Pla1 of the MTREC complex, Nature Communications, 14, 772, 2023

• RNA/DNA studies : Basic RNA/DNA isolation, PCR, RNA/DNAlibrary preparation followed by sequencing (seq.).RT-PCR, Chromatin-IP (ChIP) followed by q-PCR/seq., RNA-IP (RIP) followed by library preparation and seq. (Illumina, Oxford-Nanopore Direct RNA seq.), CRosslinking and Analysis of cDNA (CRAC).
• Protein studies: TAP-purification followed by Mass Spectrometry (MS) and analysis, Immunoprecipitation (IP), Co-IP and Western blot.
• Yeast genetic manipulation: Tetrad dissection, tagging and deletion of genes, sensitivity assays, basic plating and growing techniques.
• Drosophila studies: Handling and maintenance, ELISA- Enzyme Linked Immunosorbent Assay and Immunologic techniques.
• Acquainted with basic Molecular, Biochemical, Immunological and computational techniques (basic RNA-seq, ChiP seq analyses using Galaxy)
• Experienced in maintaining and handling human cell lines.

• Lightning talk (3 minutes) and poster presentation at EMBL Australia Postgraduate Symposium 2021- EAPS 2021.
• Panelist in the Central University of Tamil Nadu Alumni Association (CUTNAA) organized virtual panel discussion on “Opportunities abroad for higher education” held on 18th December 2021.
• Panelist in the Student IQAC organized National Webinar on “Selective degradation of genetic materials” held on 29th September 2020.
• Attended: 40th Annual Lorne Genome Conference 2019, Cumberland Lorne Resort, Victoria.
• Lightning talk (3 minutes) and poster presentation at 6th EMBL Australia PhD course held at The University of Tasmania, Hobart, Australia, 2019 (June 24- July 5).
• Attended "From Ensembles to Single-Molecule Analysis - Modern Methods of RNP Analysis 2018", Regensburg, Germany.
• Participated in 3-day workshop on ‘Emerging Trends in Chemical Sciences’ (September 21-23, 2012) organized by Department of Chemistry, Central University of Tamil Nadu.
• Attended International conference CANCERCON – 2014, held at IIT Madras from 30th-2nd Feb 2014
• Attended National conference on ‘Advances in modern biology and environment’ organized by Department of Zoology of H H Rajah’s Government College, Pudukottai, Tamil Nadu.
• Participated in National Conference on ‘Recent trends and future advances in Life Sciences’ (RFTALS) held at Central University of Tamil Nadu, India
• Participated in a National conference at St. Joseph College, Trichy on ‘Prospects and challenges in stem cell research’.

• Prof. Steven A. Johnsen, PhD, Scientific director, Robert Bosch Center for Tumor Diseases, Forschungsexzellenz des Bosch Health Campus, Auerbachstr. 112, 70376, Stuttgart, Germany, +49 711 8101-3693, steven.johnsen@bosch-health-campus.com
• A/Prof. Tamas Fischer, Associate Professor, Division of Genome Sciences and Cancer, The John Curtin School of Medical Research, Building 131, Garran Road, Canberra, ACT, 2601, Australia, +61261252194, tamas.fischer@anu.edu.au
• Prof. Thomas Preiss, Professor/ Division Head, Division of Genome Sciences and Cancer, The John Curtin School of Medical Research, Building 131, Garran Road, Canberra, ACT, 2601, Australia, thomas.preiss@anu.edu.au
• Emeritus Scientist Purnima Bhargava, Emeritus Scientist, CSIR-Center for Cellular and Molecular Biology, Hyderabad, Telangana, 500007, India, 040-27192603, purnima@ccmb.res.in
• Dr. Rippei Hayashi, Group Leader, Division of Genome Sciences and Cancer, The John Curtin School of Medical Research, Building 131, Garran Road, Canberra, ACT, 2601, Australia, rippei.hayashi@anu.edu.au