Ehsan Alirahimi

Researcher with 4+ years of experience in high content imaging and high content analysis experiments design and run as well as downstream processes such as big data analysis and visualization, target identification on primary and stable cell lines and animal model assays. Hands on experience in expression and purification of a variety of proteins from prokaryotic/eukaryotic hosts with related techniques such as cloning, in Silico sequence analysis, Ni-NTA chromatography. Eager to learn new techniques and tackle new challenges.

• Category B, Federation of European Laboratory Animal Science Associations (FELASA-B certified), 09/2022
• Good laboratory practice-GLP, 12/2021

• Research Assistant, University Hospital of Cologne, 10/2019, 06/2024, Successfully performed high content imaging and high content analysis to phenotypically screen over 120 different venoms, identifying potential pain-modulating compounds., Utilized a diverse range of in vitro assays to identify the mechanisms of action of toxins in pain sensitization signaling., Execution of various types of cellular assays using primary neural cell., Introduction of new measurements in high content imaging and high content analysis in our laboratory., Setting up new and innovative assays in close collaboration with other scientists., Preparation of research papers, reports, reviews, and summaries., Presentation of results to senior/other research staff.
• Research Assistant, Pasteur Institute of Iran, 08/2014, 09/2019, Production of different formats of antibody (ScFv, diabody, intrabody) against different targets (CTLA4, VEGFR2, PD1)., Execution of various types of functional assays using tumor cells and in vivo tumour models to validate antibodies and conjugated antibody function., Collaboration with external partners to develop and produce recombinant DNA vaccine., Successful production of Knocked-in cell line model with lentivirus gene delivery system., Screening of targets by performing page display., Successful execution of target validation on KDR cell line by application of intrabody., Successful set-up of in vivo tumour mouse model., Preparation of research papers, reports, reviews, and summaries., Presentation of results to senior/other research staff.

• Date of Birth: 03/1991
• Gender: male