Erin Noel Jordan

• Developed and implemented robust analytical methods for 52 cannabinoid-structures, validated the method for synthetic and naturally derived cannabinoids. Implemented quantitative methods via HPLC-UV and UPLC-MS/MS (qTOF).
• Developed and implemented discovery metabolomic analytical procedures using the UPLC-MS/MS (qTOF). Performed statistical, qualitative, and quantitative analysis.
• Spearheaded implementation of Oxford Nanopore Technology sequencing into the laboratory, sequenced bioengineered Saccharomyces cerevisiae genomes and transcriptomes.
• Assembled and annotated genomes for genome-wide metabolomic (GEM) in silico modeling. Developed stoichiometric constraint models (Flux Balance Analysis) and designed experiments to improve metabolic flux in bioengineered, cannabinoid-producing S. cerevisiae.
• Participated actively in regular meetings with fellow researchers to discuss project updates, challenges faced, and lessons learned during ongoing activities.
• Maintained up-to-date records of research activities and results for future reference.

● Analyzed -omics data sets including genomics, transcriptomics, and metabolomics

● Analyzed data using Bash, Python, and R

● Integrated LLM (ChatGPT) workflows into data analysis processes

● Authored and published peer-reviewed journal publications (see above).

● Established and maintained an international collaboration throughout my PhD

● Analyzed cannabinoid-glycoside potency of Pichia pastoris and plant-cell-suspension fermentation supernatants and cell pellets. Supernatants were routinely extracted with reverse-phase solid phase extraction. Cell pellets were extracted by tissue homogenization and/or sonication. Samples were analyzed by LC/MS equipped with a time-of-flight. Data analysis was performed with MassLynx and Unify Waters software-platforms.

● Contributed to downstream-processing development for large-scale extraction methods of glycosylated cannabinoids by centrifugal-partition-chromatography and column chromatography.

● Analyzed cannabinoid-potency of transformed Cannabis plants engineered with trichrome enhancement constructs and RNAi of cannabinoid-synthases with HPLC. Analyzed, reviewed, and reported data using Excel and the R statistical platform.

● Analyzed terpene content of transformed Pichia pastoris and Cannabisusing GC-FID.

● Contributed to cannabinoid-feedstock procurement with scientists and management team. Developed quality control process for cannabinoid-screening by third-party-CoA review and internal analysis.

● Developed thin-layer chromatography methods for algal wax-esters and wax-ester extraction procedures.

● Supported molecular biology research scientists by generating plasmid-constructs and performing transformations in E.coli and Agrobacterium.

● Contributed to authorship of standard-operating-procedures and experimental designs.

● Analyzed cannabinoid-potency of hemp biomass, bulk cannabinoid-rich hemp oil, and hemp-infused finished products in both water and lipid-soluble platforms. Regularly extracted samples, prepared for chromatography, performed sample analysis using HPLC, analyzed, reviewed, and reported data.

● Coordinated external-laboratory testing of other analyses including terpene, microbial, heavy metal, and pesticides

● Reviewed products for quality control for physical and sensory characteristics, created reports per standard operating procedures, collaborated with quality assurance/material review as necessary

● Created novel formulations and formulation procedures for phytocannabinoid-rich/zero-THC hemp-infused finished products including both water-soluble and oil-soluble platforms

● Contributed to research and development of new QC analysis procedures as well as manufacturing practices and procedures

● Conducted stability studies of bulk products and novel formulations

● Reported all results in a timely manner and communicated effectively with managers and supervisors throughout all processes; Worked under deadlines

● Trained and mentored new chemists and laboratory technicians

● Kept administrative records for budgeting, inventory, chain of custody, safety, out-of-specification trends, and control charts

● Supervised and maintained inventory

● Reared Drosophila arizonae and D. mojavensis flies, prepared food, orchestrated crosses.

● Genotyped flies using PCR/gel electrophoresis, coordinated external- Sanger sequencing

● Created single-guide RNA’s for CRIPSR-Cas9 non-homologous end-joining pathway knockdown generation and for homology directed-repair knock-in generation

● Created homology directed repair templates (pUC19 vector, single-stranded oligonucleotide donor templates)

● Analyzed data, drew conclusions and evaluated experimental outcomes, and maintained written records of procedures

● Sterilized materials, practiced general lab upkeep and hygiene

• Instructed labs and administered grades for the biology 1000 – evolution and ecology course.

• Privately instructed high school and collegiate general biology courses.

• Graded written work, including final exam papers.