Henrikje Wilhelmina Smits
life scientist
Rees
Übersicht
9
9
years of professional experience
8
8
years of post-secondary education
Berufserfahrung
Research assistant at the department of Horticulture & Product Physiology
Wageningen University & Research
Wageningen
2023.04 - 2025.12
PhD candidate at Prof Dr Bettina Hause lab
Leibniz Institute of Plant Biochemistry
Halle (Saale)
2018.04 - 2022.06
- Characterization of the interplay of auxin and jasmonates in tomato flower development and fruit set
- Methods: LC-MS measurements of phytohormones, PEG-embeddings, semi-thin sectioning & immune stainings, RNA-seq & transcriptome analysis, ChIP-Seq, qPCR, Golden-Gate & Gateway cloning, laser scanning microscopy, CRISPR/Cas9 mediated genome editing, tomato transformation, plant tissue culture
- Supervisor: Prof Dr Bettina Hause
Internship
DuPont Industrial Biosciences
Wageningen
2016.11 - 2017.05
- Targeted integration by homologous recombination in M.thermophila
- Methods: Fungal transformation, Southern blot, enzyme assays
- Examiner: Prof Dr Willem van Berkel
- Supervisors: Dr Caroline Michielse & Henriek Beenen
Ausbildung
University entrance diploma -
St.-Georg-Gymnasium
BocholtMSc. - Molecular Life Sciences, Physical Biology
Wageningen University (WUR)
Wageningen 2014.11 - 2017.12
BSc. - Molecular Life Sciences, Human and Animal Physiology
Wageningen University
Wageningen 2009.09 - 2014.10
Kompetenzen
- German (mother tongue)
- English (fluent)
- Dutch (fluent)
- Microsoft Office
- R programming (basics)
Sprachen
German (mother tongue)
English (fluent)
Dutch (fluent)
Benutzerdefiniert
- Research assistant at HPP, 04/01/23, Present, General lab tasks like chemical waste disposal, ordering, management of glycerol stocks etc., Establishment & management of standard operating procedures (SOPs), Supervision of students
- PhD project at Leibniz Institute of Plant Biochemistry, Halle (Saale), 04/01/18, 06/30/22, Characterization of the interplay of auxin and jasmonates in tomato flower development and fruit set, Methods: LC-MS measurements of phytohormones, PEG-embeddings, semi-thin sectioning & immune stainings, RNA-seq & transcriptome analysis, ChIP-Seq, qPCR, Golden-Gate & Gateway cloning, laser scanning microscopy, CRISPR/Cas9 mediated genome editing, tomato transformation, plant tissue culture
- Internship at DuPont Industrial Biosciences, Wageningen, 11/01/16, 05/31/17, Targeted integration by homologous recombination in M.thermophila, Methods: Fungal transformation, Southern blot, enzyme assays
- Minor thesis at the chair group 'Plant Developmental Biology', Wageningen University, 06/01/16, 10/31/16, Creating Arabidopsis knock-out mutants involved in lateral root formation by multiplex CRISPR/CAS9 and comparing sgRNA efficiency in vivo and in vitro, Methods: Golden Gate Cloning, Agrobacterium-mediated plant transformation (N.tabacum & A.thaliana), CRISPR/Cas9
- Master thesis at the Laboratory of Biochemistry, Wageningen University, 10/01/15, 05/31/16, Investigation of the homodimerization of the DNA-binding domain of the auxin response factor 5 (ARF5/Monopteros) by FRET-FLIM experiments, Methods: Cloning (SLiCE, LIC cloning, site-directed mutagenesis), protoplast isolation, protoplast transfection, confocal microscopy, fluorescence lifetime imaging microscopy, protein expression (E.coli), SDS-PAGE, Western blotting
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Zeitleiste
Research assistant at the department of Horticulture & Product Physiology
Wageningen University & Research
2023.04 - 2025.12
PhD candidate at Prof Dr Bettina Hause lab
Leibniz Institute of Plant Biochemistry
2018.04 - 2022.06
Internship
DuPont Industrial Biosciences
2016.11 - 2017.05
MSc. - Molecular Life Sciences, Physical Biology
Wageningen University (WUR)
2014.11 - 2017.12
BSc. - Molecular Life Sciences, Human and Animal Physiology
Wageningen University
2009.09 - 2014.10
University entrance diploma -
St.-Georg-Gymnasium