JELENA JEREMIĆ

Investigation of macrophage trace elements and metallome dynamics during bacterial exposure using inductive-plasma mass spectrometry (ICP-MS) and X-ray Imaging (XRI).

• Developed standardized protocols for sample preparation and correlative imaging workflows.
• Implemented calibration standards for quantitative image analysis, leading to identification of key mechanisms in macrophage- induced bacterial intoxication.
• Automated image analysis, reducing manual analysis time by 50% and enhancing reproducibility.
• Designed and functionalized imaging holders to improve cell adherence for detailed cellular interaction studies.
• Supervised and trained 2 master students to thesis completion.

Super Resolution Imaging of bacterial virulent complexes in cryo conditions (cryo-SR).

• Optimized super resolution microscopy to maintain biocompatibility and enhance imaging precision under low temperatures.
• Achieved 10-fold improvement in resolution and signal-noise ratios, making a foundational framework for in situ analysis of an important vaccine target.
• Implemented cryo-SR techniques with accessible, commercially available optical equipment.
• Collaborated in a multi-disciplinary team ensuring alignment on objectives and timely project completion.