Khanyile Sibanda

• Project: Direct reprogramming of hypothalamic astrocytes into neurons using CRISPR activation in vitro
• Techniques: Molecular Cloning, Primary astrocyte cell culture and maintenance, Hek293 and Neuronal2A cell culture, RT-qPCR, Immunohistochemistry, Immunocytochemistry, Western Blot, Confocal imaging
• Sucessfully showed the increased expression levels of target neuronal genes of 12 created plasmids

• Project: Quantifying pancreatic cancer components to uncover potential biomarkers crucial for early detection and targeted interventions in this aggressive cancer type.
• Techniques: 3D organoid Cell culture, RNA extraction, Immunostaining, ELISA, RT-qPCR

Project: Exploring the effects of mutation in a putative peptide transporter in the root nodules of the L.japonicus plant

Techniques: Extraction of nodules from plant, flow cytometry, acetylene reduction assay, confocal microsopy imaging

• Assisted faculty in laboratory research and experiments.
• Managed grading and data entry for coursework and exams.
• Stayed current on lab techniques and safety protocols for enhanced procedural efficiency.
• Provided student support, including answering queries and offering feedback.

• Project involved the Isolation of Cryptococcus neoformans from the environment for medical and molecular mycology analysis
• Roles: Aseptic techniques, culture maintenance, media isolation from environmental sources, project planning and time management