Manfred Anim

• Conducted postdoctoral research on Hepatitis delta virus (HDV), focusing on identifying immunological parameters that could define Pegylated interferon alpha (Peg-IFNα) treatment response in chronic HDV infection
• Developed a flow cytometry panel to characterize anti-IFNα autoantibody neutralization capabilities
• Performing single-cell RNA/TCR/CITE and Spatial transcriptomics sequencing.
• Designed experiments using sophisticated statistical techniques, yielding robust results that supported key hypotheses.
• Enhanced research efficiency by optimizing experimental designs and protocols.
• Contributed significantly to breakthrough discoveries through expert data analysis and interpretation skills.
• Worked as member of integrated project team in highly collaborative work environment.

Delivered lectures on the mechanisms, diagnosis, and treatment of primary immunodeficiency (PAD) biannually to ten to fifteen PhD students.

• Developed and presented course materials tailored to advanced graduate students.
• Engaged students through interactive discussions and case studies.
• Presented complex concepts in clear and relatable manner, simplifying difficult material for better comprehension by diverse audiences.
• Enhanced student understanding by incorporating real-world examples and case studies into lectures.
• Developed strong rapport with students, creating a supportive learning environment that encouraged active participation.
• Continuously updated course materials based on student feedback and emerging research, ensuring relevance and accuracy of content presented.

• Wrote research papers, reports, and summaries regarding primary antibody deficiencies (PADs)
• Designed experiments using targeted next-generation, ectopic gene expression, western blot, quantitative real-time Polymerase chain reaction (qRT-PCR), flow cytometry and confocal microscopy techniques, yielding robust results that supported key hypotheses.
• Evaluated new technologies for potential integration into existing research processes, driving continuous improvement efforts.
• Mentored junior scientists, fostering professional growth and enhancing team capabilities.
• Increased research collaboration with interdisciplinary teams, leading to more comprehensive findings.
• Presented research findings at international conferences, increasing visibility and recognition within the field.
• Advanced scientific understanding within the field by publishing impactful peer-reviewed articles.

• Studied the function of highly conserved sequences within MEIS1 by employing CRISPR-Cas9 to delete this conserved element
• Adapted quickly to new areas of study within the internship program, proving versatility as a researcher.
• Maintained detailed records of experimental procedures and findings, ensuring reliability and reproducibility of results.
• Participated actively during meetings by sharing ideas and providing constructive feedback on ongoing projects.
• Collaborated with faculty advisors to refine hypotheses, improving overall project direction and focus.
• Developed strong problem-solving skills by identifying challenges during experiments and proposing effective solutions.
• Presented research findings to faculty members, effectively communicating complex concepts in an understandable manner.
• Attended meetings and presentations to learn project details.
• Visualised research findings through data tables, graphs and presentations.
• Supported fellow interns with their projects, fostering collaboration and teamwork within the group.

• Conducted thorough experiments following established protocols while accurately documenting results for future reference.
• Increased efficiency in lab operations by maintaining a clean and organized work environment while adhering to safety protocols.
• Managed project deadlines effectively by prioritizing tasks according to urgency and importance.
• Developed strong problem-solving skills through troubleshooting experimental challenges as they arose during the research process.
• Recorded experimental findings in laboratory notebook to analyze and interpret experimental findings.
• Collected research data through experimentation, surveys and leading focus groups.

• Planned, modified, and executed research techniques, procedures, and tests
• Re-establishing extracellular matrix for in-vitro pancreatic islets culture and using phospho-histone H3 as a biomarker for pancreatic islets proliferation
• Isolation of pancreatic islets and culturing
• Basal and stimulated glucose-insulin secretion
• Protein extraction from pancreatic islets
• Determination of insulin concentration using ELISA assay technique
• Confocal microscopy image processing and analysis.

• Supported research and development efforts to get new insights into the multifunctional roles of a novel Plasmodium falciparum Armadillo-Type Repeat Protein
• Recombinant technology for protein synthesis using a bacterial expression system
• Protein purification using Ion Exchange Chromatography and Affinity Chromatography
• Western Blot
• Immunoprecipitation.
• Enhanced research outcomes by conducting thorough literature reviews and data analysis.
• Assisted in the publication process for academic journals, editing manuscripts and ensuring proper formatting guidelines were met.
• Developed skills in various research methodologies, ensuring high-quality work across diverse projects.
• Contributed to the completion of multiple research projects, managing time effectively to meet deadlines.
• Maintained detailed records of all experiments conducted, ensuring easy accessibility and proper documentation procedures were followed.
• Strengthened collaboration between team members, maintaining open communication channels for better project results.

• Set up visual aids, equipment, and classroom displays to support the lecturer's lesson delivery
• Assisted lecturers with classroom management and document coordination to maintain a positive learning environment
• Recorded grades for coursework and tests in the online reporting system
• Tutored struggling students individually and in small groups to reinforce learning concepts
• Partnered with laboratory technicians to organize research experiments following the school's curriculum, goals, and objectives
• Worked flexible hours: night, weekend, and holiday shifts.