Naailah Beebee Nessah ROHEEMUN

• Perform High-throughput laboratory processes using automated equipment in Histology (Tissue processor for fixation & dehydration; embedding into paraffin blocks; sectioning using a microtome & cryostat; staining on Ventana/ Leica Bond platforms, coverslipping, slide scanning using Axio Scanner)
• Assay establishment of emerging biomarkers/targets (IHC & IF), fit for duplex portfolio
• Establishment & Implementation of new IHC Assays (Mouse on Mouse Methodologies as the Innovation aspect)
• Ensuring and adhering to FAIR (Findability, Accessibility, Interoperability, Reuse) data princples in operating digital pathology platforms (OncoData, SLIMS, Arvados, PMA & Archiving system)
• Data Analysis using Visiopharm, AI based quantification software
• Currently learning spatial relationship between neighborhood cell populations (R based program)
• Report Writing based on Scientific interpretation
• Liaising with Pharmacology Leaders and Stakeholders on a daily basis

• Isolation of hair follicles
• Cryosection preparation of skin and hair follicles samples
• Performing different histochemical staining (such as Ki67, Caspase 3, Alkaline Phosphatase, CK8, CK18, CK20, MMP-1 and Collagen-1)
• Successfully completed projects on wound healing, atopic dermatitis, alopecia areata and skin aging
• DNA extraction & PCR
• Isolation of different compartments of hair follicles using laser capture microdissection (LCM), followed by RNA extraction for RNA sequencing
• Maintaining general laboratory organization & maintenance of equipment
• Collaborating in scientific laboratory meetings
• Training of new colleagues

• Use of innovative protocols set up in house to substantiate new claims
• Conducted clinical trial studies (Phase II-III) according to ICH-GCP
• Writing up of study plan and study report- act as study personal
• Independent & precise setting up of study file specific for each study
• Set up TMF (Trial Master File)
• Designed CRFs (Case Report Forms) both in English and French
• Successfully liaised with sponsors during the course of a study
• Provided support in audit’s inspection
• Extensive use of cell culture for safety and efficacy testing (e.g MTT for cytotoxicity)
• Ex-Vivo assays, ELISA’s using human skin explants and hair
• Delivered oral presentations

• I have worked in Haematology and Blood Bank departments
• Duties included:
• Performed blood & urine analysis (FBC, Differential count, ESR, PBS, microscopic analysis)
• Blood Grouping and Rh typing & cross-matching
• Ran QC’s and performed maintenance on analysers
• Liaised with doctors to inform about patient’s critical values.

• Received, separated, logged and stored biological samples correctly on LIMS
• Writing up of study-specific laboratory instructions
• Answered telephone enquiries and dealt with them professionally.
• Performed routine maintenance and calibration of analysers, balance and reviewed quality control results before running samples.
• Gained and applied scientific knowledge to recognise incorrect results and take remedial action.

• My sandwich placement revolved around a multidisciplinary laboratory whereby maintaining good working relations with all staff and promoting effective teamwork and working under pressure
• Received, separated, logged and stored biological samples correctly on LIMS
• Writing up of study-specific laboratory instructions
• Answered telephone enquiries and dealt with them professionally
• Performed routine maintenance and calibration of analysers, balance and reviewed quality control results before running samples
• Validated an immunoassay of total 25 (OH) Vitamin D on the UnicelDxI analyser & comparison with the ClinRep 25-OH-Vitamin D2/D3 UPLC assay on the WATERS ACQUITY UPLC H-class system, as part of my Bachelor's theisis