Nadja Bücklein

• Pizza delivery driver at Joey's Pizza Service in Ludwigshafen (Sep 2012 - Oct 2013)
• Voluntary six-week internship at the kindergarten "Von-Weber-Straße" in Ludwigshafen (Feb - Apr 2013)
• Farmhand/Cook at a sheep and cattle station in Queensland, Australia (Oct 2015 - Jan 2016)
• Cleaning/Supervisor at myHome in Melbourne, Australia (Feb 2016 - Jun 2017)
• Order Picking at Medpex in Ludwigshafen (Aug - Oct 2017)
• Private Tutor for chemistry and mathematics (8th - 11th grade) (Mar 2018 - Sep 2019)
• Bartender at MS Connexion Complex Mannheim (Apr 2018 - Mar 2020)
• Bartender at Nelson Bar and Café (Aug 2022 - Oct 2023)
• Student assistant in project evaluation in the "Transfer Together" project at PH Heidelberg (Sep 2022 - Nov 2022)
• Bartender/Service at Tap Room Jungbusch (Okt 2023 - Sep 2024)
• Full-time Waitress/Eventmanagement at bootshaus Mannheim - Restaurant | Events (Juli 2025 - March 2026)

Biochemistry & Protein Analysis

• DNA isolation, hydrolysis and HPLC analysis
• Protein isolation and purification, including egg albumin
• Protein quantification: Lowry, BCA, Bradford, UV absorption
• Protein electrophoresis: albumin/egg albumin electrophoresis and SDS-PAGE
• Protein molecular-weight determination by gel chromatography and SDS-PAGE
• Isoelectric focusing
• Enzyme activity assays
• ELISA, FACS, SPR, Western blotting
• Proximity Ligation Assay (PLA) and imaging
• Immunological test-strip development
• Tollens' reagent

Molecular Biology & Genetic Engineering

• Plasmid DNA minipreparation
• PCR and gel electrophoresis
• Cloning and restriction analysis
• Transfection and transduction
• Southern blotting
• DNA sequencing, including pyrosequencing

Cell Culture & Cell-Based Assays

• Mammalian cell culture: seeding, splitting and maintenance
• Development and optimization of cell-based potency and functional assays
• Antibody-dependent phagocytosis assays
• Bioassay development and execution
• Cell-based fluorescence assays

Extracellular Vesicles & Proteomics

• Extracellular vesicle (EV) isolation, purification and characterization
• EV protein extraction and sample preparation
• EV cargo and protein analysis
• Proteomic sample preparation
• Mass spectrometry: MALDI-TOF, MS/MS

Microscopy & Image Analysis

• Fluorescence, confocal and brightfield microscopy
• Fluorescence imaging and image acquisition
• Quantitative image processing and analysis using Fiji/ImageJ

Animal Research & Histology

• Mouse colony care and handling
• C57BL/6J mouse experimental work
• Treadmill-based exercise studies and critical speed (CS) testing
• Tissue collection and preparation
• PFA fixation/washing, paraffin embedding, microtome sectioning
• Histochemical staining

Microbiology

• Bacterial culture, isolation and enumeration
• Determination of bacterial counts in water
• Isolation of pure cultures from mixed cultures
• Gram staining and KOH string test
• Bacterial growth kinetics
• Spore constituent isolation and spore staining
• Solid culture and enrichment media preparation
• Macroscopic and microscopic microbial characterization
• Quantitative and qualitative determination of airborne microorganisms
• Antibiotic inhibition zone assays
• Disinfectant efficacy testing
• Microbial cell counting

Analytical, Organic & Physical Chemistry

• Chromatography: HPLC, GC, column chromatography, TLC
• Mass spectrometry: MALDI-TOF, MS/MS
• Photometry, titration and complexometry
• Ion-selective electrode measurements
• Kjeldahl analysis
• Buffering systems
• Extraction, purification, synthesis and distillation
• Lipid extraction
• Adsorption isotherms
• Combustion calorimetry
• Determination of dissociation constants and isoelectric points
• First- and second-order reaction kinetics
• Vapor-pressure osmometry, freezing-point measurements and molecular-mass determination by osmometry

Laboratory Instrumentation & Quality

• Bioreactor instrumentation
• Laboratory blood dialysis machine operation
• GMP/GLP
• Clean-room environment
• Antibody production
• Scientific writing and documentation