Nigar Manafova

Supervisor:

PD Dr.Florence Bareyre

Research project for Master's thesis:

Studying the role and composition of A1 and A2 type astrocytes in spinal cord injury for different treatment groups using spinal cord organoids as a model system

Learned skills:

• Human iPSC culture
• Organoid generation
• Cryosectioning of mouse tissue and organoids
• Immunohistochemistry
• Imaging (mainly Confocal)
• Image analysis

Supervisor:

Dr.Magdalena Murawska

Research project:

Studying the role of the human histone chaperone FACT in mitosis

Learned skills:

• Human cell culture
• Microscopy (SD confocal) and image processing
• Western blot and SDS-PAGE
• Gene knockdown
• Transfection
• Drug treatments of human cell lines
• Wrote reports to summarise data and implications of results

Supervisor:

Prof. Dr. Jovica Ninkovic

Research project:

Glioblastoma Multiforme (GBM) and reactive gliosis in human cerebral organoids

Learned skills:

• Bioimaging with confocal microscopy
• Immunohistochemistry
• Introduction to iPSC culture
• Wrote reports to summarise data and implications of results

Supervisor:

Assoc. Prof. Dr. Isik G. Yulug

Project:

Done detailed literature research and wrote a review article about the subject "Circadian clock in health and disease: the genes that play role in breast cancer"

CENTRAL VETERINARY LABORATORY

• Completed general training program for molecular biology laboratory techniques and biosafety
• Gained hands-on experience in serological methods in a virology laboratory
• Was introduced to the bacteriology lab of the molecular biology department and observed how bacteria enrichment is done
• Learned about procedures and protocols applied to food safety evaluation

Used techniques:

• ELISA
• qPCR
• Nuclec acid extraction
• Sample handling