Pouria Bahrami
Postdoctoral Scientist
Cologne
Summary
- Interdisciplinary molecular biologist with strong expertise in biotechnology, mycology, protein characterization, and cell/tissue culture.
- Skilled in managing and analyzing structured biological datasets, with hands-on experience in scientific IT platforms and digital lab notebooks; currently learning R for enhanced data handling.
- Scientist with proven track record of impactful research and scientific contributions. Adept at designing and conducting complex experiments, contributing to advancements in field. Known for strong collaboration with team members and adaptability to changing research needs, ensuring successful project outcomes. Key skills include molecular biology techniques and scientific communication.
- Over four years of entrepreneurial experience as co-founder of a biotech company, with proven strengths in project management, marketing, scientific support, and stakeholder communication.
- Robust practical knowledge of genetic engineering techniques in fungi, bacteria, and yeast, including DNA construct design, heterologous protein expression, purification (hands in FPLC), and post-translational modifications (PTMs).
- Self-driven and adaptable team player with a strong problem-solving mindset, excellent interdisciplinary communication skills, and the ability to independently manage complex research projects across academic and applied settings.
Overview
12
12
years of professional experience
3
3
Languages
Work History
Postdoctoral Scientist
University Of Cologne (AG Zuccaro)
04.2024 - Current
- Analyze molecular interaction mechanism of beneficial and pathogenic microbes with host.
- Molecular studies of B. sorokiniana triggered programmed cell death in their host and non-adopted hosts.
- Establishing B. sorokiniana protoplast transformation and genetic manipulation technique.
- Molecular and cellular characterization of Bipolaris sorokiniana secreted Anti-Microbial Peptides (AMPs) in host and model systems.
- Developed new experimental methods to address specific research questions, resulting in more accurate data collection.
- Maintained detailed documentation of all experiments conducted, enabling reproducibility of results and fostering transparency in research practices.
- Enhanced collaboration between researchers by organizing seminars and workshops on relevant topics.
Visiting Scientist
Uni. Bremen, Bimol. Interaction Center, Islet Lab
03.2023 - 02.2024
- Exploring the impact of an exogenous expression of a kinase activator protein on mammalian model systems with a pharmaceutical perspective for cancer treatment.
- Designing the project focused on the exogenous microbial-secreted enzyme expression triggering cell apoptosis.
- Developing in-vitro and in-vivo assays to analyze cellular and molecular responses on the exogenous protein expression.
- Presented scientific papers at department meetings and symposia.
Scientific Assistant (PhD)
University Of Bonn, INRES
10.2020 - 03.2023
- Deciphering filamentous fungi-secreted enzyme targets highly conserved kinases to suppress cell wall expansion.
- Designed and led a primary project focused on identifying the secretory protein and its host molecular targets involved in infection under sterile conditions.
- Developed in-vitro and in-vivo phenotypical, functional assays and molecular experiments to study pathogen-host interaction in the model systems, including S. cerevisiae, U. maydis, E. coli, HeLa, HEK293T, A. thaliana, N. benthamiana, M. polymorpha.
- End-to-end successful characterization of fungal-secreted protein, a novel interacter in the host, mechanism of action, downstream changes, and consequences of the expression.
- Collaboration with internal and external collaborators and projects.
- Training of lab members and visiting scientists during their onboarding time.
- Optimized experimental design through thorough background research and collaboration with subject matter experts.
Junior Scientific Assistant
Leibniz Institute of Plant Genetics and Crop Plant
03.2019 - 09.2020
- Managing GMO biological samples libraries.
- Screening 258 fungal secreted proteins in the non-host system.
- Training of lab members and visiting scientists during their onboarding time.
Co-founder
Kian Tajhiz Azma
01.2015 - 01.2019
- Management, Marketing and scientific support of molecular biology materials.
- Grew the customer base through targeted outreach and networking events.
- Marketing and technical support for molecular biotechnology products, Kits, and chemicals.
- Presenting new kits, products and techniques.
- Set up the new molecular laboratories and training.
- Biological materials and laboratory equipment
Obligatory military service
Khanavadeh Hospital
01.2013 - 01.2014
- Education and research department of Khanevadeh Hospital (Army ground force Hospital)
Education
Master of Science - Biotechnology
University of Tehran
Tehran, Iran 1 2010 - 1 2012
BSc - Plant Protection
University of Tabriz
Tabriz, Iran 01.2005 - 1 2009
Ph.D. - Molecular Biology
University of Bonn
Bonn, Germany 01.2020 - 1 2024
Skills
Intellectual curiosity
Critical and innovative thinking
Interdisciplinary collaboration
Team work and collaborative
Entrepreneurship and management
undefinedTechnical Skills
- Cell culture, Cell assays, Cell transformation, Cryopreservation of Fungi (Ascomycetes, Basidiomycetes), Bacterial (Pseudomonas, E. coli), S. cerevisiae, Plant (A. thaliana, N. benthamiana, Z. mays, H. vulgar, M. polymorpha), Mammalian (HeLa, Hek293T),
- DNA/RNA extraction, cDNA synthesis, PCR, RT/qPCR, midi/mini prep, reporter assay, Golden Gate, Gibson, Crispr, Homologous recombination, classical cloning and transformation, Biolistic bombardment,
- Recombinant protein expression and purification, Kinase assay, PTM, ELISA, Immunoprecipitation, Western Blotting, Proximity labeling (TurboID), Fluorescence microscopy, Digital microscopy, Cell imaging and analysis,
- Virus-induced gene silencing (VIGS)
Training Skills
- 2020-2025, Project/thesis supervisor MSc students and training of Scientists at onboarding time at the University of Bonn
- 2015-2019, Training of technicians and students on new products and molecular biology lab equipment
Other Skills
- Germany Driving Licence, Class B.
- Member of the organization team of Biotrophy workshop-2022, ButanTagung-2022.
- 'Leadership Skills for the Non-Academic Job Market' workshop as part of Ph.D. Plus project at Uni-Bonn in 2023.
- “From Idea to Pitch” Young Entrepreneurs in Science workshops at Uni. Cologne July 2024.
- Office package (word processing, spreadsheet, and presentation software), hands-on CLC, Geneiuos, Alphafold, R, Inkscape.
- Work in the sales department and involvement in scientific groups have allowed me to develop strong communication abilities. I am capable of effectively engaging with others, expressing ideas, and conveying information in a way that is easily understood.
Interests
Sports
Economy
Reading
Psychology
Conference Presentations
- Pouria Bahrami, Nyasha Charura, Alga Zuccaro, 10/11/2024, Tissue-specific colonization strategies of Bipolaris sorokiniana in adapted and non-adapted plant hosts., EMBO-Workshop, Sant Feliu de Guixols, Spain
- Mamoona Khan, Pouria Bahrami, 02/06/2023, Growth hormone targeting effectors of a gall-forming fungus - Ustilago maydis., MBP-2023
- Kishor D. Ingole, Pouria Bahrami, Nithya Nagarajan, & Armin Djamei, 08/28/2022, Characterization of dual functional fungal effector ApoE1 to establish biotrophy in maize., Botanik-Tagung 2022
- Nithya Nagaranjan, Kishor D. Ingole, Pouria Bahrami, & Armin Djamei, 08/28/2022, An experimental study on a family of effectors from Ustilago maydis in non-host model plants reveals evolutionarily conserved targets., Bontanik-Tagung 2022
- Pouria Bahrami, & Armin Djamei, 04/06/2022, Screening for effectors with conserved host targets in a non-host plant., Biotrophy Workshop 2022
Selected Publications
- Geravandi, S., Liu, H., Pahwa, H., Krishna Madduri, M., Atawneh, F., Miraki, A., Elisabeth Kruf, A., Khazaei, M., Bahrami, P., Gotti, D., Rafizadeh, S., Elawour, M., Maria Grasso, A., Bund, D., Lupse, B., Azizi, Z., Zabad, O., Bouzakri, K., Horwitz, M., … Ardestani, A., 2025, The Hippo terminal effector YAP boosts enterovirus replication in type 1 diabetes., Nature Communication, Accepted
- Bahrami, P., Ingole, K., de Sousa Teixeira E Silva, N., Khan, M., Cortes Roman, T., & Djamei, A., 2024, Fungal effector targets plant GSK3 kinases to manipulate the plant cell expansion., New Phytologist, under revision
- Oliver Kayser, Wim J. Quax, 2015, Medicinal Plant Biotechnology from basic research to the industrial application, 1 ed, ETKA, 333, 3527314431, Book (translated in Persian from English)
Timeline
Postdoctoral Scientist
University Of Cologne (AG Zuccaro)
04.2024 - Current
Visiting Scientist
Uni. Bremen, Bimol. Interaction Center, Islet Lab
03.2023 - 02.2024
Scientific Assistant (PhD)
University Of Bonn, INRES
10.2020 - 03.2023
Ph.D. - Molecular Biology
University of Bonn
01.2020 - 1 2024
Junior Scientific Assistant
Leibniz Institute of Plant Genetics and Crop Plant
03.2019 - 09.2020
Co-founder
Kian Tajhiz Azma
01.2015 - 01.2019
Obligatory military service
Khanavadeh Hospital
01.2013 - 01.2014
BSc - Plant Protection
University of Tabriz
01.2005 - 1 2009
Master of Science - Biotechnology
University of Tehran
1 2010 - 1 2012
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