Public speaking oratory skills recognized as > 47 public speaking awards
Direct report management : 2 technicians and BD team trainings
Presentation skills
Data analytics and Molecular biology tools
Microsoft Word
Santosh Krishna Subramanian
Scientific Specialist Biologics
Hofheim am Taunus,Hessen
Summary
Experienced preclinical scientist specializing in biologics within the fields of Inflammation, Autoimmunity, and Cancer. Recognized scientific expert in TNF, cytokine biology, and inflammatory pathways such as NFkB, MAPK, and Wnt. Proficient in various methodologies including immunoblots, Flow cytometry, MSD/Luminex assays, cell-based assays, and Immunology assay development. SME at Eurofins for the past 5 years, expanding knowledge in biologics discovery across multiple therapeutic areas. Eager to leverage expertise, confidence, and motivation in a new role to contribute to novel drug/biologics discovery efforts with a strong team spirit and positive attitude.
Overview
23
23
years of professional experience
9
9
years of post-secondary education
5
5
Languages
Work History
Scientific Expert : Biotherapeutics & Immunology
Eurofins Discovery
Planegg
01.2020 - Current
- Technical SME for the Biologics discovery, Bioanalytics, protein production, Bioassays, Immunology services and Immunosafety.
- Supporting commercial team, providing scientific proposals, pricing, negotiations and active Business Development from lead to post sales
- Representing Eurofins Biotherapeutics in Conferences and meetings
- Training and development of BD Team and supporting marketing efforts
- Used strong analytical and problem-solving skills to develop effective solutions for challenging situations.
Research Scientist (Metabolic Diseases and Inflammation)
Evotec International GmbH
Göttingen
01.2018 - 01.2019
- Managed Virtual Biotech
- In vivo pharmacology scientist for NASH & Liver diseases
- Platform lead for Biomarkers – MSD, Luminex and ELISA
Postdoctoral Scientist Autoimmune Diseases
Sanofi Aventis Deutschland GmbH
Frankfurt Am Main
01.2015 - 01.2018
- Preclinical RnD in Inflammation and Immunology.
- Anti-cytokine, cell death inhibitors and multi-specific biologics
- TNF super family SME
- Primary cell-based assays and Multiplex biomarker discovery for RA.
- Immune cell assays for POC and MOA of small molecule cytokine inhibitors
- In vivo mice models for RA (CIA, CAIA)
- CRO Collaboration (Evotec) for MS based biomarker discovery
- Supervision of dedicated technical staff and student trainees
Postdoctoral Scientist
CECAD, University of Cologne
Cologne
01.2011 - 01.2015
- Role of Cell death (TNF superfamily, NFkB and RIPK1) using several Knockout and transgenic mice models, Primary hepatocytes, immunohistochemistry, FACS and biochemical methods investigating responsible pathways affecting Liver inflammation, NASH and HCC-development.
- Primary investigation of chronic inflammation in Liver and gut axis in connection with absence of NFkB signalling, dissecting parenchymal contribution to varying Inflammatory or oncological outcomes
- Investigated TAK1, NEMO, NFkB and Fibrotic pathways using exvivo liver tissues, primary hepatocyte culture and bile eithelial cells
Doctoral Student
Charité Campus Benjamin Franklin
Berlin
01.2007 - 01.2010
- Role of UDCA / bile acids in preventing Intestinal Inflammation and colon cancer
- Used mice, cell-based assays, Affymetrix microarray analysis, si/shRNA, Adeno-viral approaches, western Blotting, Fluorescence microscopy, over-expression and knock-downs to investigate Inflammatory, Oncology pathways specially MAPK, Wnt and NFkB signalling & immunological Cell death of gut epithelia.
- Awarded PhD with “Magna cum laude” in April 2011
Senior Research Scientist (Molecular Biology-Pharmacology)
Nicholas Piramal Research Centre
Mumbai
01.2005 - 01.2007
- Specialist for recombinant protein expression and purification facility for BL21DE3 based system
- (Facility setup from scratch, sourced and setup ACTA Prime methods and several columns for SEC, Affinity and other methods
- In-house high purity protein supplier lab for cytokine inhibitor screening platform
- Develop & perform cytotoxic and Reporter assays for screening of small molecules and supporting HTS group
Research Associate - Biosimilars
Wockhardt Research Centre
Aurangabad
01.2002 - 01.2005
- Construct design, cloning and transfections in BL21De3, Yeasts and CHO lines for biologics and Biosimilars
- Cell Line engineering of yeast strains Hansenula polymorpha, Pichia pastoris and BL-21De3 for expression of Insulin analogs.
- Produced Master and Working cell banks and QC documentation for producer clones for Yeast and BL21De3 following ICH-guidelines
- Pilot scale Multi vessel fermentation, HPLC and biochemical analysis of peptides and inclusion body refolding and a full analytics of biologics with ICH-qualified methods
- QA documentation report generation
Education
Ph.D. - Biochemistry And Oncology
Charité Campus Benjamin Franklin
Berlin 01.2007 - 01.2011
Master of Science - Biotechnology
Dr. GRD College of Science
Coimbatore, India 01.2000 - 01.2002
Bachelor of Science - Microbiology
St. Joseph’s College
India 01.1997 - 01.2000
Skills
Work Visa
Germany, Niederlassungserlaubnis (Permanent)
Work Experience
20 years
Personal Information
- Date of Birth: 12/30/79
- Nationality: Indian
- Marital Status: Married
Publications
- Cell Death Differ., 12/01/19, 26, 12, 2710-2726
- Cancer Cell, 11/01/15, 28, 5, 582-598
- Cell Death and Differentiation, 11/01/14, 21, 11, 1721-1732
- Plasma Chemistry and Plasma Processing, 08/01/13, 33, 5, 921-940
- International Journal of Cancer, 06/01/12, 130, 12, 2771-2782
- European Journal of Cancer Prevention, 09/01/12, 21, 5, 413-422
Disclaimer
I hereby declare that all the information that I provide here is true to the best of my knowledge.
Distinctions
- Developed from scratch BL21De3 producer MCB for Insulin analogs, optimizing the complete cycle of Biologics development from clone to Biology ready Protein. MCB currently in commercial production.
- Best Post Doc Poster award at Sanofi, Frankfurt - 2017
- FELASA-B (European standard for animal work) certification for Laboratory animal work
- Promotionsstipendium der Berliner Krebsgesellschaft e.V.
- PCCC Grant from Helmholtz alliance for cancer research and DFG funded research projects
- Dr. Falk foundation e.V. grant and Deutsche Krebsgesellschaft, Germany
- More than 45 awards for public speaking, extempore and debates during career.
Timeline
Scientific Expert : Biotherapeutics & Immunology
Eurofins Discovery
01.2020 - Current
Research Scientist (Metabolic Diseases and Inflammation)
Evotec International GmbH
01.2018 - 01.2019
Postdoctoral Scientist Autoimmune Diseases
Sanofi Aventis Deutschland GmbH
01.2015 - 01.2018
Postdoctoral Scientist
CECAD, University of Cologne
01.2011 - 01.2015
Doctoral Student
Charité Campus Benjamin Franklin
01.2007 - 01.2010
Ph.D. - Biochemistry And Oncology
Charité Campus Benjamin Franklin
01.2007 - 01.2011
Senior Research Scientist (Molecular Biology-Pharmacology)
Nicholas Piramal Research Centre
01.2005 - 01.2007
Research Associate - Biosimilars
Wockhardt Research Centre
01.2002 - 01.2005
Master of Science - Biotechnology
Dr. GRD College of Science
01.2000 - 01.2002
Bachelor of Science - Microbiology
St. Joseph’s College
01.1997 - 01.2000
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