Shannon Brianna Do Rosario
Frankfurt am Main
Summary
Molecular biotechnology graduate with over five years of laboratory experience in molecular biology, genetics, and cell culture. Proficient in classical and advanced techniques, including nanopore sequencing, PCR, primer/sgRNA design, and mammalian cell culture. Known for reliability and adaptability in meeting dynamic team needs. Exhibits strong organizational abilities and effective communication skills. Skilled in data analysis, troubleshooting, and delivering accurate results in both research and clinical laboratory settings
Overview
4
4
years of professional experience
Work History
Intern/Master thesis
Sartorius Stedim Cellca GmbH
01.2025 - 09.2025
- Focused on nanopore sequencing and experimental method development.
- Prepared comprehensive reports summarizing data analysis findings, informing key decision-makers of important trends and patterns.
- Gained hands-on experience in primer and sgRNA design and validation, mammalian cell culture (including fed-batch cultivation, stock culture maintenance, and cell bank preparation), and DNA extraction.
- Responsibilities also included sample preparation, data analysis, and contributing to optimization strategies in an industrial research setting.
- Analyzed problems and worked with teams to develop solutions.
Working student- Laboratory Assistant
Urologische Praxis Dr. Marlies Franke
04.2024 - 12.2024
- Responsible for urine sample collection and analysis, centrifugation, microscopy, rapid diagnostic tests, sterilization of instruments, documentation, and assisting the physician during procedures.
- Also supported front-desk reception work.
- Mentored lab assistants on proper handling practices for hazardous materials while maintaining compliance with environmental regulations.
- Improved lab organization and inventory management through meticulous record-keeping and maintenance of supplies.
Medical Technical Assistant
Gemeinschafts Praxis Pathologie
05.2023 - 10.2023
- Handled incoming tissue samples, assisted during grossing, embedded samples in paraffin wax, and performed microtomy and histological staining procedures.
- Gained exposure to cytology and pathology workflows.
Intern- Medical Laboratory Technician
Dr. Raghuwanshi’s Diagnostic Centre and Pathology Laboratory
09.2022 - 10.2022
- Performed routine blood and urine testing, ECGs, sample collection, and assisted in ultrasound procedures.
Intern- Medical Laboratory Technician
Manipal Hospitals
08.2021 - 09.2021
- Worked across multiple departments including histopathology, microbiology, haematology, serology, biochemistry, and clinical pathology.
- Performed tissue staining, microtomy, and blood collection.
Education
Master of Science - Molecular Biotechnology
Johannes Gutenberg University
Mainz09-2025
Bachelor of Science - Biotechnology
Dhempe College of Arts And Science
Miramar, Goa06.2022
Secondary and Higher Secondary School Certificate - undefined
Our Lady of the Rosary High School and Higher Secondary School
Dona Paula, Goa04.2019
Skills
- Nanopore Sequencing
- Mammalian cell culture
- Fed-batch cultivation
- Stock culture splits
- Cell bank preparation
- DNA extraction
- single guide RNA and primer design
- PCR
- nrLAM-PCR, primer design
- Gel electrophoresis
- Streaking
- Pour and spread plating
- Bacterial quantification
- Microbiological assays
- Staining techniques
- Preparation of culture media
- Preparation of laboratory buffers and solutions
- Lab equipment calibration
- Quantitative analysis
- Aseptic techniques
- Work under safety cabinets
- Sample handling
- Documentation, data analysis, torubleshooting
- Optimization strategy
- MS Word, PowerPoint, Excel
- Geneious Prime
- Strong communication
- Independent work capability
- Teamwork and collaboration
- Attention to detail
- Quick learner
- Multitasking and organization
Personal Information
- Date of Birth: 20.10.2001
- Nationality: Portuguese
- Marital Status: Single
Training
- Explore and Understand DNA, BioEra Life Sciences, R&D Centre, Pune, India, 2-day workshop on DNA isolation and agarose gel electrophoresis.
- National Webinar on “Biosafety and Biosecurity”, Department of Biotechnology, Dhempe College of Arts and Science, Goa, India, Training program on Nano Biomedicine.
- Training program on Nano Biomedicine, Makeintern & E-Cell IIT Kharagpur, Dhempe College, India, 2-day workshop with practical application in the synthesis of nanoparticles and applications in nanobiomedicine.
Languages
English
Bilingual or Proficient (C2)
German
Upper intermediate (B2)
Hindi
Bilingual or Proficient (C2)
Hobbies
Photography
Reading
Travelling
Timeline
Intern/Master thesis
Sartorius Stedim Cellca GmbH
01.2025 - 09.2025
Working student- Laboratory Assistant
Urologische Praxis Dr. Marlies Franke
04.2024 - 12.2024
Medical Technical Assistant
Gemeinschafts Praxis Pathologie
05.2023 - 10.2023
Intern- Medical Laboratory Technician
Dr. Raghuwanshi’s Diagnostic Centre and Pathology Laboratory
09.2022 - 10.2022
Intern- Medical Laboratory Technician
Manipal Hospitals
08.2021 - 09.2021
Secondary and Higher Secondary School Certificate - undefined
Our Lady of the Rosary High School and Higher Secondary School
Master of Science - Molecular Biotechnology
Johannes Gutenberg University
Bachelor of Science - Biotechnology
Dhempe College of Arts And Science
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