Carmen Piirsalu
Munich
Overview
4
4
years of professional experience
1
1
Certification
Work History
Master Thesis
Department of Medical Biochemistry & Microbiology
01.2024 - 06.2024
Investigation into CD44-hyaluronan signaling in Palbociclib-resistant triple-negative breast cancer
Junior Researcher
GPCR Workgroup, Chair of Bioorganic Chemistry
06.2022 - 08.2022
Design and generation of recombinant baculoviruses, multiprotein co-expression in mammalian cells using baculovirus expression vectors
Bachelor Thesis
GPCR Workgroup, Chair of Bioorganic Chemistry
01.2021 - 06.2022
Development of a MultiBacMam™ technology-based assay to study ligand binding to Frizzled-receptors
Summer Internship
Microbiology Laboratory of Valio
06.2020 - 08.2020
Microbiological analysis of dairy products
Education
M.Sc. Biochemistry -
Uppsala University
Uppsala, Sweden06-2024
B.Sc. Chemistry, cum laude -
University of Tartu
Tartu, Estonia06.2022
Skills
- Cell Culturing (2D & 3D culturing, cancer cells and primary fibroblasts)
- Cell-Based Methods (generation of transient overexpression cell lines, viability/proliferation assay, FACS)
- Live-cell imaging and cell staining
- PCR Techniques (qPCR, RT-PCR)
- Western Immunoblotting
- Molecular cloning
- Protein Purification (IMAC, SEC, IEC)
- Biophysical Methods (ITC, DSF, CD)
- Interaction Validation Methods (phage display, ELISA, FRET, Co-IP)
- Software (Microsoft Office, GraphPad Prism, ImageJ)
- Modeling Programs (PyMol, Coot)
Certification
- LAS Certificate in Laboratory Animal Science
ENGAGEMENT
- Acknowledged for the help with molecular cloning in the paper llluminating Neuropeptide Y Y4 Receptor Binding: Fluorescent Cyclic Peptides with Subnanomolar Binding Affinity as Novel Molecular Tools
- FEBS3+ conference of Estonian, Latvian and Lithuanian Biochemical Societies, Poster Presentation: Frizzled-receptor systems – lightening the black box with fluorescence methods, Tallinn, Estonia, 06/2022
- Specialisation stipend from the European Regional Development Fund (1000€), 01/2022
- 34th China Adolescence Science & Technology Innovation Contest, Poster Presentation: Genetic Testing for Lactose Intolerance, Macau, China, 07/2019
- Estonian National Contest of Young Scientists, a special award to represent Estonia at an international scientific contest, 04/2019
- Member of Biochemistry Journal Club, Institute of Chemistry, University of Tartu, 01/2021-05/2022
- Member or Tumor and Cell Biology Journal Club, Department of Medical Biochemistry and Microbiology, Uppsala University, 01/2024-06/2024
PERSONAL INFORMATION
Nationality: Estonian
Personality: Ambitious, curious, well-organized, friendly
Hobbies: Running, swimming
Languages
English
Advanced
C1
German
Elementary
A2
Estonian
Bilingual or Proficient (C2)
Timeline
Master Thesis
Department of Medical Biochemistry & Microbiology
01.2024 - 06.2024
Junior Researcher
GPCR Workgroup, Chair of Bioorganic Chemistry
06.2022 - 08.2022
Bachelor Thesis
GPCR Workgroup, Chair of Bioorganic Chemistry
01.2021 - 06.2022
Summer Internship
Microbiology Laboratory of Valio
06.2020 - 08.2020
B.Sc. Chemistry, cum laude -
University of Tartu
M.Sc. Biochemistry -
Uppsala University
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