Kaushik Sheladiya

Topic- Evaluation of PCR-based detection methods for different small RNAs in plants

• Project aims to evaluate plethora of PCR-based detection methods for plant small RNAs, for exemplary constitutively expressed (housekeeping) miRNAs ath-MIR159a and bdi-miR159a-3p by measuring efficiency, specificity, sensitivity, and quantification confidence of each method. PCR, qPCR, RT and PAGE molecular techniques are used.

Topic - Plant breeding Intern on Maize

• Demonstrated adaptability by quickly learning new concepts and techniques as required for different assignments within Breeding program
• Seed cleaning, counting, sorting, labeling and seed treatment
• Opening cobs for scoring and harvest
• Performed disease scoring (Fusarium, root and stock lodging, helmintho sporium), Processing and analysis of scored data, silage samples collection
• Digital Phenotyping with Drone
• Attending theoretical and practical breeding courses, understanding of Limagrain software for genomic selection, prediction, validation, phenotyping, envirotyping and breeding database software as well as Spotfire software for data visualization.

Topic- RNAi Technology (RNA Interference)

• Molecular biology: RNA isolation, cDNA synthesis, PCR
• Established RNAi Insect bioassays
• Performed Insect Micro-Injection
• Support in conducting field and greenhouse trials, insect rearing and plant cultivation

• Worked effectively in fast-paced environments.
• In master's thesis, I have studied proteins and hormones responsible for Parkinson's disease in Drosophila melanogaster. Further, I gain experience to work with insects and flies