Summary
Overview
Work History
Education
Skills
Awards
Publications
References
Expertiseskills
Timeline
Generic

Lieu Dang

Postdoctoral Researcher
Heidelberg

Summary

With over seven years of experience in protein chemistry and drug discovery, specializing in protein purification, biochemical and biophysical characterization. Skilled in mass production of protein in milligram scale, high-throughput screening, and protein expression. Proficient in Python for data analysis and experienced in cross-functional collaborations. Strong leadership, communication, and project management skills demonstrated through mentorship and teamwork in international research environments.


Overview

2025
2025
years of professional experience
11
11
years of post-secondary education

Work History

PostDoc Researcher

DKFZ-ZMBH Alliance
Heidelberg
5 2024 - Current
  • Conceptual design and coordinate projects
  • Supervise PhD and Master students

Doctoral Researcher

DKFZ-ZMBH Alliance
Heidelberg
09.2019 - 05.2024
  • Characterize mode of action of promising-small molecule hits that inhibit the human disaggregation systems
  • Perform in-vitro high throughput screening of small molecule inhibitors targeting neurodegenerative diseases
  • Purify and conduct biochemical and biophysical analysis of misfolded/aggregated human protein sorting factors

Research Assistant

Hong Kong University of Science and Technology
Hong Kong
02.2017 - 02.2019
  • Purify and perform a biochemical analysis of human DGCR8 protein in Pasha complex, focusing on its role in miRNA biogenesis
  • Establish a mammalian suspension−cultured system for efficient protein expression

Education

Ph.D. - Biochemistry

DKFZ-ZMBH Alliance, Heidelberg University
Heidelberg
09.2019 - 05.2024

Master of Science - Life Science

Hong Kong University of Science And Technology
Hong Kong
02.2017 - 02.2019

Bachelor of Science - Biology

VNU Hanoi University of Science
Hanoi, Vietnam
09.2012 - 10.2016

Skills

Protein purification

SDS-PAGE

Native-PAGE

HPLC analytics

LC/AKTA

Immunoassay-based methods

Sucrose gradient ultracentrifugation

FRET-enzymatic assays

Fluorescence intensity

Fluorescence polarization assay

Luminescence assay

Absorbance

Static light scattering

Dynamic light scattering

Nano different scanning fluorimetry

Thermal Shift assay

Isothermal Titration Calorimetry

Biolayer interferometry

Assay development

Large-scale recombinant protein production

Mode of action characterization

PCR

Restriction-free cloning

Gibson Assembly

In vitro miRNA synthesis

Small RNA-cDNA generation

Supervision

Teaching

Teamwork

Communication

MS Office

ImageJ

GraphPad Prism

Data Analysis with Python

Pandas

Numpy

Matplotlib

Awards

  • 2017, 2019, HKUST Postgraduate Research Scholarship award, 2 years
  • 2015, DGIST − Korea Research Scholarship
  • 2012, 2016, VNU Scholarship for Outstanding Academic Achievement, 4 years
  • 2012, Bronze prize in Vietnam National Biology Olympiad
  • 2011, Golden prize in Hanoi Biology Olympiad

Publications

  • G Zoltsman, T.L Dang, M Kuchersky, O Faust, M Silva, T Ilani, A.S Wentink, B Bukau, R Rosenzweig, A unique mechanism in Class A JDPs recognizes and stabilizes mutant p53, Molecular Cell, 04/18/24, https://doi.org/10.1016/j.molcel.2024.02.018
  • T.L Dang, C.T Le, M.N Le, T.D Nguyen, T.L Nguyen, S Bao, S Li, T.A Nguyen, Select amino acids in DGCR8 are essential for the UGU-pri-miRNA interaction and processing, Communications Biology, 3, 344, 2020, https://doi.org/10.1038/s42003-020-1071-5
  • T.A Nguyen, J Park, T.L Dang, Y.G Choi, V.N Kim, Microprocessor depends on hemin to recognize the apical loop of primary microRNA, Nucleic Acids Research, 46, 11, 06/20/18, 5726-5736, https://doi.org/10.1093/nar/gky248

References

  • Prof. Dr. Bernd Bukau, ZMBH − Heidelberg University, Germany, bukau@zmbh.uni-heidelberg.de, +49-6221 54 6795
  • Prof. Dr. Matthias Mayer, ZMBH − Heidelberg University, Germany, m.mayer@zmbh.uni-heidelberg.de, +49-6221 54 6829
  • Dr. Anne Wentink, Leiden University, Netherlands, a.s.wentink@lic.leidenuniv.nl, +31 71 527 4534

Expertiseskills

Protein purification: more than 30 proteins purified (Chaperones, p53, Pasha proteins) including protein complex (Drosha/DGCR8, 120 kD) and insoluble proteins (mutant p53 proteins, small heat shock proteins) from E.coli, mammalian cell HEK293E/T., SDS-PAGE, Native-PAGE, HPLC analytics, LC/AKTA, Immunoassay-based methods, Sucrose gradient ultracentrifugation, Biochemical methods: FRET-enzymatic assays, Fluorescence intensity and Fluorescence polarization assay, Luminescence assay, Absorbance, Biophysical methods: Static/Dynamic light scattering (SLS/DLS), Nano different scanning fluorimetry (nanoDSF), Thermal Shift assay (TSA), Isothermal Titration Calorimetry (ITC) and Biolayer interferometry (BLI), Assay development for automation technology screening, Large-scale recombinant protein production, Mode of action characterization: small molecule inhibition mechanism using protein biochemistry techniques above, Standard and alternative cloning methods: PCR, restriction-free cloning, Gibson Assembly, In vitro miRNA synthesis and purification, Small RNA-cDNA generation, Supervision and teaching: Technical supervision and mentoring bachelor/master students, Teaching assistant for practical courses, Teamwork: Experienced in collaborative international projects and multidiscipline working teams, Communication: Regularly presented research findings to diverse audiences, MS Office, ImageJ and GraphPad Prism, Data Analysis with Python (Pandas, Numpy, Matplotlib), English (professional working proficiency), German (conversant), Vietnamese (native)

Timeline

Ph.D. - Biochemistry

DKFZ-ZMBH Alliance, Heidelberg University
09.2019 - 05.2024

Doctoral Researcher

DKFZ-ZMBH Alliance
09.2019 - 05.2024

Master of Science - Life Science

Hong Kong University of Science And Technology
02.2017 - 02.2019

Research Assistant

Hong Kong University of Science and Technology
02.2017 - 02.2019

Bachelor of Science - Biology

VNU Hanoi University of Science
09.2012 - 10.2016

PostDoc Researcher

DKFZ-ZMBH Alliance
5 2024 - Current
Lieu DangPostdoctoral Researcher