Vahid Fallahzadeh
Plant Biochemist
Tübingen
Summary
Highly skilled researcher with extensive experience in cloning, expression of recombinant proteins, protein purification, biochemical analysis, and laboratory research. Expertise in FPLC, HPLC (affinity, gel filtration, ion exchange, reverse-phase chromatography), and gas chromatography. Adept at optimizing purification protocols, troubleshooting analytical instruments, and working in fast-paced laboratory environments. Strong sense of responsibility, reliability, and structured independent work ethic. Currently improving German language proficiency for better integration into the professional environment.
Overview
16
16
years of professional experience
Work History
Postdoctoral Researcher
Zentrum Für Molekularbiologie Der Pflanzen (ZMBP)
05.2023 - Current
- Presented research findings at international conferences, fostering knowledge exchange among peers.
- Designed effective experimental controls, ensuring robust conclusions from research studies.
- Developed novel methodologies for data analysis, significantly improving research efficiency.
- Cloning receptor kinase from plants and heterologous expression of these proteins in prokaryotic cells
- Developed purification protocols for secreted bacterial proteins and peptides.
- Utilized chromatography techniques (HPLC, FPLC) to separate and analyze bioactive compounds.
- Managed laboratory operations, ensuring compliance with safety and quality control standards.
Sabbatical Research Fellowship
Western Sydney University, Australia
06.2018 - 02.2019
- Purified and characterized elicitor proteins from microbial pathogens for application in plant resistance studies.
- Developed a gas chromatography-based screening method for pathogen resistance in crop breeding.
- Designed and implemented protein purification techniques for biochemical assays.
Teaching
Azarbaijan Shahid Madani University
01.2014 - 04.2023
- "Plant Diseases" for second year Plant Protection students, 2015-2016
- "Pathogenic Viruses and Prokaryotes of Plants" for third year Plant Protection students, 2015-present
- "Physiology of Plant Parasitism" for first year Master's students, 2015-2016
- "Management of Plant Diseases" for forth year Plant Protection students, 2016-present
- "Important Diseases of Fruit Trees", 2016-present
- "Pathogenic Prokaryotes in Plants" for first year Master's students, Azarbaijan Shahid Madani University, 2015-present
- "Principles of Biochemistry" for second year Plant Protection students, 2015-present
PhD project
Zentrum für Molekularbiologie der Pflanzen (ZMBP)
01.2011 - 06.2013
- My Ph.D. research at the University of Tübingen focused on characterizing microbial elicitors and their interactions with plant receptor-like proteins, requiring precise protein isolation and characterization.
Research assistant
Azarbaijan Shahid Madani University
07.2011 - 12.2011
- Our aim in this project was to isolate and screen flourescent pseudomonads from different fields of north west of Iran to find out plant beneficial isolates
- We succeed to find some 2,4-diacetylphloroglucinol and phenazine producing isolates via molecular and biochemical approaches
- In this project I gained valuable experience in purification of antibiotics by HPLC
Research assistant
University of Tehran
10.2008 - 10.2009
- During this period, I was participated in a project which aimed to optimize growth culture composition and condition for commercial production of beneficial bacteira and siderophore as a biofertilizer
- In this research I had the chance to get acquainted to operate bench top fermentors
Education
Ph.D. - Plant pathology
Tehran University-University of Tübingen
Tehran, Iran-Tübingen, Germany 04.2001 - 01.2009
Master of Science - Plant Pathology
University of Tehran
Tehran, Iran 04.2001 -
Bachelor of Science - Plant Protection
University of Mohaghegh Ardabili
Ardabil, Iran 04.2001 -
Skills
- Biochemical assays: ELISA, SDS-PAGE, Western blot, enzyme activity assays, kinase assays based on antibody detection and radiography, purification of organic compounds such as antibiotics
- Analytical techniques: Gas Chromatography (GC), preparative column chromatography,Protein purification: HPLC, FPLC (affinity, ion exchange, size-exclusion, reverse-phase chromatography)
- Molecular biology: Cloning based on Gate way and Golden gate systems, QPCR, plasmid preparation, DNA/RNA extraction, Protoplast extraction and transfection
- Microbial techniques: Bacterial protein expression, fermentation technology
- Instrumentation: Operation and troubleshooting of laboratory equipment including fermentors and chromatography systems
- Software: GraphPad Prism, Geneious Prime, CLC Main Workbench, MS Office
- Currently studying German to enhance communication in professional settings
- Strong ability to work independently and in collaborative environments
- Experience in troubleshooting and optimizing laboratory protocols
Selected Talks
- The Arabidopsis TNL CONSTITUTIVE SHADE AVOIDANCE 1 (CSA1) reveals activation of PTI and ETI responses downstream of the pattern recognition co-receptor BAK1, Lion, France, 2023
- Fermentation technology and its application in production of biocontrol agents, Karaj, Iran, 2008
- Phase Variation in Bacteria (with emphasis on Pseudomonas spp.), Karaj, Iran, 2010
Awards
- Iranian Ministry of science Scholarship for performing PhD project abroad, 2012-2014
- Rank 9th among 2000 participants in Iranian graduate nationwide entrance exam of Phytopathology, 2006
- Member of the Iran's National Elites Foundation (INEF), 2010-2014
Publications
Fallahzadeh-Mamaghami V, Weber H & Kemmerling. B. 2023. BAK-up: the receptor kinase BAK-TO-LIFE 2 enhances immunity when BAK1 is lacking. Stress Biology, 3: 42.
Fallahzadeh-Mamaghani V., Mikhael M., Plett J. 2023. Chickpea pathogen-resistance-rating correlates with responsiveness to an immune elicitor protein from Phytophthora medicaginis. Rhizosphere. 25: 100654.
Fallahzadeh-Mamaghani V., Golchin S., Shirzad A., Mohammadi H., Mohammadivand F. 2021. Characterization of Paenibacillus polymixa N179 as a robust and multipurpose biocontrol agent. Biological Control. 154: 104505.
Fallahzadeh-Mamaghani V. 2016. Proteome analysis of Xanthomonas citri subsp. citriprotein extract with elicitor activity on Arabidopsis thaliana. Genetic Engineering and Biosafety Journal 4(2): 113-123. (in Persian with English abstract)
Jehle AK., Lipschis M., Albert M., Fallahzadeh-Mamaghani V., Fürst U., Mueller K. and Felix G. 2013. The Receptor-like Protein ReMAX of Arabidopsis thaliana Detects the novel MAMP emax from Xanthomonas. The Plant Cell 25: 2330-2340.
Shirzad A., Fallahzadeh V. and Pazhouhandeh M. 2012. Antagonistic Potential of Fluorescent Pseudomonads and Control of Crown and Root Rot of Cucumber Caused by Phythophtora drechsleri. The Plant Pathology Journal. 28: 1-9
Fallahzadeh V., Ahmadzadeh M., Marefat A., Ghazanfary K. 2010. Application of rhizobacteria for induction of systemic resistance to bacterial blight of cotton caused by Xanthomonas campestris pv. malvacearum by fluorescent pseudomonads of rhizosphere. Journal of Plant Protection Research. 4: 416-420.
Fallahzadeh V., Ahmadzadeh M. and Sharifi R. 2010. Growth and pyoverdine production kinetics of Pseudomonas aeruginosa 7NSK2 in an experimental fermentor. Journal of Agricultural Technology 6: 107-115.
Fallahzadeh V., Ahmadzadeh M., Sharifi R. 2009. Screening systemic resistance inducing fluorescent pseudomonads for control of bacterial blight of cotton caused by Xanthomonas axonopodis pv. malvacearum. Journal of Plant Pathology. 91: 731-738.
Ashofteh F., Ahmadzadeh M., Fallazadeh V. 2009. Effect of mineral components of the medium used to grow biocontrol strain utpf61 of Pseudomonas fluorescens on its antagonistic activity against Sclerotinia wilt of sunflower and its survival during and after the formulation process. Journal of Plant Pathology. 91: 669-675.
Sharifi R. Ahmadzadeh M. Sharifi-Tehrani A. Fallahzadeh V. 2009. Competition for iron uptake by fluorescent pseudomonads to control Rhizoctonia Solani Kühn, a causing agent of bean damping-off disease. Journal Of Agriculture Science and Technology 22:183-196. (in Persian with English abstract)
Student Supervision
- Eric Helios, Bachelor’s, University of Tübingen, 2024
- Shadi Dastan, Master's, ASMU, 2020-2022
- Samira Samadzadeh, Master's, ASMU, 2020-2022
- Sima Delkhahnia, Master's, ASMU, 2016-2018
- Jamileh Hasanoghlu, Master's, ASMU, 2016-2018
- Simin Nasiri, Master's, ASMU, 2015-2017
- Samaneh Ghasemzadeh, Master's, ASMU, 2015-2017
- Samaneh Golchin, Master's, ASMU, 2015-2017
- Hosein Abassi, Master's, ASMU, 2014-2016
- Roya Shahbazi, Master's, ASMU, 2014-2016
- Samira Keighobadi, Master's, ASMU, 2013-2015
- Fatemeh Abdoli, Master's, ASMU, 2013-2015
Timeline
Postdoctoral Researcher
Zentrum Für Molekularbiologie Der Pflanzen (ZMBP)
05.2023 - Current
Sabbatical Research Fellowship
Western Sydney University, Australia
06.2018 - 02.2019
Teaching
Azarbaijan Shahid Madani University
01.2014 - 04.2023
Research assistant
Azarbaijan Shahid Madani University
07.2011 - 12.2011
PhD project
Zentrum für Molekularbiologie der Pflanzen (ZMBP)
01.2011 - 06.2013
Research assistant
University of Tehran
10.2008 - 10.2009
Ph.D. - Plant pathology
Tehran University-University of Tübingen
04.2001 - 01.2009
Master of Science - Plant Pathology
University of Tehran
04.2001 -
Bachelor of Science - Plant Protection
University of Mohaghegh Ardabili
04.2001 -
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