Dr. Maryam OMIDI
Postdoctoral Researcher
Alsterkrugchaussee 583a, 22335, Hamburg
Summary
More than 18 years of research experience in different institutes and universities:
- > 5 years as a postdoctoral researcher: 1.5 years in downstream processing, and from June 2020 in upstream and downstream processing, Hamburg, Germany.
- 6 years in analytical chemistry -LC-MS/MS & chromatography) during PhD and after that in Hamburg, Germany.
- 6 years in the field of upstream and downstream processing (gene cloning, protein expression, purification) and about one year in the field of molecular biology in Tehran, Iran
Overview
21
21
years of professional experience
11
11
years of post-secondary education
3
3
Languages
Work History
Postdoctoral Researcher
Institute of Osteology and Biomechanics, Skeletal Pathobiochemistry, AG Prof. Dr. Pohl, Universitätsklinikum Hamburg-Eppendorf
06.2020 - Current
- Structural analysis of proteins related to rare-bone-diseases via in vivo crystallisation
- Designed, developed, wrote, and reviewed methods/SOP/protocols
- Sample preparation and measurement via LC-MS/MS analysis (identification and quantification) & data analysis & interpretation of the data from MS analysis
- Cell culture (mammalian, insect cells)
- Gene cloning, PCR, qPCR
- SDS-PAGE, Western Blot, Enzyme Assay
- Supervising students and new lab mates
- The projects are funded by Die Deutsche Forschungsgemeinschaft -DFG-.
Postdoctoral Researcher
Institute Of Clinical Chemistry And Laboratory Medicine, Laboratory Of Molecular Inflammation, AG Dr. Fuchs, Universitätsklinikum Hamburg-Eppendorf
07.2018 - 12.2019
- Method establishment and development for purification of proteins (with more than 99% purity) as new medicinal proteins
- Enzyme assay Western blot of purified proteins
- Liophoilisation, Ultra & Dia- filtration of purified proteins prior to Test-incetjion to animals
- Neutrolis, Cambridge, Massachusetts, United States funded the project.
Language Course
Deutsch
11.2017 - 06.2018
Deutsch (Niveau C1), UNS - Deutsch für Studium und Beruf
Research assistant
Institute of Clinical Chemistry and Laboratory Medicine, Mass Spectrometric Proteomics, AG Prof. Dr. Schlüter, Universitätsklinikum Hamburg-Eppendorf
11.2012 - 11.2017
- Investigation of the Impact of Magnesium Implants on the Proteomes of Bone Cells and Bone Tissue
- Method establishment for preparation of bone samples for the measurement via LC-MS/MS
- Sample preparation of tissues using picosecond infrared laser -PIRL- for Proteome analysis
- Sample preparation and method development of cancer tissues for measurement via LC-MS/MS & MALDI-Imaging
- Protein purification via ÄKTA
- Supervision of Bachelor/Master students
- The projects were funded by Virtual Institute -VI-, Helmholtz-Zentrum, Geesthacht, and the SUREPIRL project -ERC advanced grant), Hamburg, Germany.
Language Course
English And Deutsch
12.2010 - 11.2011
English (IELTS) and Deutsch (till B1)
Research assistant
Tehran University of Medical Sciences, School of Medicine, Tehran, Iran
05.2009 - 12.2010
- Laboratory assistant in molecular biology techniques such as PCR, cloning,...
Research assistant
Protein Chemistry Unit & Protein Purification Lab, Biotechnology Department, Pasteur Institute of Iran
03.2003 - 03.2009
- Purification of recombinant and native proteins via FPLC & HPLC
- SDS-PAGE & Western blot
- Optimization of methods for protein purification via different chromatography techniques
- Participating in workshops as a theoretical and practical trainer.
Education
Ph.D. - Mass Spectrometry-Based Proteomics
University of Hamburg
University Medical Center Hamburg-Eppendorf (UKE) 04.2012 - 04.2017
Master of Science -
Alzahra University
05.2003 - 10.2006
Bachelor of Science (BSc), Cellular and Molecular biology - Microbiology -
Azad University
10.1999 - 06.2002
Skills
Chromatography -HPLC & FPLC-undefined
Publications
- Targeting NETs using dual-active DNase1 variants. Front Immunol. 2023 May 23;14:1181761., 2023, https://pubmed.ncbi.nlm.nih.gov/37287977/
- Investigation of the impact of magnesium versus titanium implants on protein composition in osteoblast by label free quantification. Metallomics. 2020 Jun 24;12(6):916-934., 2020, https://pubmed.ncbi.nlm.nih.gov/32352129/
- Proteome analysis of human mesenchymal stem cells undergoing chondrogenesis when exposed to the products of various magnesium-based materials degradation. Bioact Mater. 2019 Apr 24;4:168-188., 2019, https://pubmed.ncbi.nlm.nih.gov/31049466/
- Ultrafast extraction of proteins from tissues using desorption by impulsive vibrational excitation. Angew Chem Int Ed Engl. 2015 Jan 2;54(1):285-8., 2015, https://pubmed.ncbi.nlm.nih.gov/25346525/
Volunteer Experience
Attended as a theoretical and practical trainer in some workshops in Germany & Iran such as:
- “Proteomics” (2014), Hamburg, Germany.
- “An Introduction to Basic Methods in Biotechnology” (included DNA and RNA Methods, Upstream Processing, Downstream Processing and Protein Analysis) at Biotechnology Department, Pasteur Institute of Iran (2005-2007), Tehran, Iran.
- “Amino Acid analysis workshop (Electronic& Practical)” (February 2006) at Biotechnology Department, Pasteur institute of Iran in Tehran and Cinnagen Company, Karaj, Iran.
Timeline
Postdoctoral Researcher
Institute of Osteology and Biomechanics, Skeletal Pathobiochemistry, AG Prof. Dr. Pohl, Universitätsklinikum Hamburg-Eppendorf
06.2020 - Current
Postdoctoral Researcher
Institute Of Clinical Chemistry And Laboratory Medicine, Laboratory Of Molecular Inflammation, AG Dr. Fuchs, Universitätsklinikum Hamburg-Eppendorf
07.2018 - 12.2019
Language Course
Deutsch
11.2017 - 06.2018
Research assistant
Institute of Clinical Chemistry and Laboratory Medicine, Mass Spectrometric Proteomics, AG Prof. Dr. Schlüter, Universitätsklinikum Hamburg-Eppendorf
11.2012 - 11.2017
Ph.D. - Mass Spectrometry-Based Proteomics
University of Hamburg
04.2012 - 04.2017
Language Course
English And Deutsch
12.2010 - 11.2011
Research assistant
Tehran University of Medical Sciences, School of Medicine, Tehran, Iran
05.2009 - 12.2010
Master of Science -
Alzahra University
05.2003 - 10.2006
Research assistant
Protein Chemistry Unit & Protein Purification Lab, Biotechnology Department, Pasteur Institute of Iran
03.2003 - 03.2009
Bachelor of Science (BSc), Cellular and Molecular biology - Microbiology -
Azad University
10.1999 - 06.2002
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