Ramsha Masood
Hamburg,Germany
Summary
Dedicated individual eager to contribute to team success. Quick-learner with knowledge of lab compliance requirements and safety protocols. Monitors requirements to support compliance.
Overview
12
12
years of professional experience
9
9
years of post-secondary education
Work History
Scientific researcher
Laboratory for Translational Research at Department for Internal Med 1. University Medical Center Hamburg Eppendorf
Hamburg, Germany
04.2023 - Current
- Designed and executed experiments focused on cancer genetics, translating basic scientific discoveries into clinical applications.
- Performed DNA and RNA extraction, PCR, and dPCR to analyze genetic materials relevant to cancer studies.
- Conducted research using ctDNA extraction techniques, contributing to advanced cancer diagnostics.
- Utilized cell biology techniques, including tissue culture and cell assays, to study cellular responses.
- Applied Western blotting, immunohistochemistry, and flow cytometry to analyse protein expressions in cancer biology.
- Isolation of extracellular vesicles from serum of patients receiving immunotherapy.
- Performed legend plex immune maker assay to validate the presences of immune checkpoint on surface of vesicles.
- Collaborated with clinicians, biostatisticians, and researchers to integrate findings into clinical research projects.
- Maintained and calibrated laboratory equipment to ensure accuracy in experimental outcomes.
- Provided guidance to students and new staff, ensuring adherence to ethical guidelines and institutional regulations.
Biologist
Exosomes and Tumor biology laboratory, Institute for Infection Prevention and Hospital Epidemiology, Universität Klinikum Freiburg Germany
Freiburg, Germany
04.2021 - 02.2023
- Handled specimens and conducted tests according to established protocols, keeping efficient records on all experiments
- Conducted research on specific Sars-CoV-2 human serum samples by isolating and characterizing nanoparticles that includes viral particles, extracellular vesicles and protein
- Conducted NTA-based biomarker detection and validation using patient samples, aiding in cancer diagnosis.
- Detection of polydispersity and variability of nanoparticles by NTA, DLS
- Developed protocols for tissue culture and cell assays to study cancer cell behavior.
- Performed DNA extraction and conducted both real-time and standard PCR assays for tumor biology research
- Carried out day-day duties accurately and efficiently
- Used critical thinking to break down problems, evaluate solutions and make decisions.
Research Assistant
Exosomes and Tumor biology laboratory, Institute for Infection Prevention and Hospital Epidemiology, Universität Klinikum FreiburgGermany
Freiburg, Germany
03.2020 - 03.2021
- Worked both independently and collaboratively in fast-paced laboratory environment
- Performed statistical, qualitative and quantitative analysis
- Attended seminars and symposiums to improve overall knowledge and understanding
- Gathered, arranged and corrected research data to create representative graphs and charts highlighting results for presentations.
Biologist
Dr. Shamsi & Ansari Diagnostics
Karachi, Pakistan
05.2012 - 05.2013
- Worked in different departments which include Biochemistry, Transfusion medicine, Microbiology and Molecular biology
- Managed day to day activities of lab and provided both assistance and supervision to students performing research for projects
- Designed molecular or cellular laboratory experiments, did execution and interpreted results
- Hands-on participation in the execution of GMP batches, including supporting cleaning & steam- in-place activities
- Support Equipment Scale-up activities and equipment commissioning including creation of standard operating procedures.
Education
International Double Master’s in Biomedical Science (M.Sc.) - Biomedical Sciences
Albert-Ludwigs University of Freiburg, Germany And University of Buenos Aires, Argentina
Freiburg Germany 02.2018 - 02.2020
Diploma of Advanced Studies in Biomedical Science - Biomedical Sciences
Albert-Ludwigs University of Freiburg
Freiburg, Germany 10.2018 - 02.2019
Bachelors in Biomedical sciences - Biomedical Sciences
Dow Medical University
Karachi, Pakistan 01.2009 - 07.2013
High School - Biology
St. Joseph’s College For Women
Karachi, Pakistan 09.2006 - 09.2008
Skills
- Graphpad prism
- R programming
- Python programming
- Microsoft packages
- Excellent communication skills
- Ability to work under pressure
- Target oriented and self-motivated
- Team player
- Isolation and characterization of Cells, Viral particles and Exosomes
- Size exclusion chromatography
- Differential Ultracentrifugation
- Detection of protein expressions
- ELISA
- Western blot
- Nanoparticle Tracking Analysis
- Dynamic light scattering analysis of nanoparticles
- Cell Culture techniques
- PBMcs isolation
- DNA/RNA extraction
- Quantitative PCR analysis
- Packaging of Viruses
- Flow cytometry
- Multiplex bead assisted flow cytometry
- Immunofluorescence
- Immunoassays
- Writing Reports
- Presenting at Conferences
- Confocal microscopy
- Transmission electron microscopy
Accomplishments
- Oral presentation “Immune checkpoint profiles on circulating extracellular vesicles predict response to immunotherapy in hepatocellular carcinoma” at The International Liver Congress 2024, organized by the European Association for the Study of the Liver (EASL), June 2024; Milan, Italy
- Poster presentation “Assessment of extracellular vesicles in pancreatic cancer patient samples for the detection of tumor specific biomarkers” at International Society of Extracellular Vesicles (ISEV) Conference; May 2022; Lyon, France
- Academic Excellence Award, German Academic Exchange Services DAAD, for outstanding student during MSc
Languages
English
Fluent
German
Intermediate
Spanish
Elementary
Urdu
Native
Arabic
Intermediate
Publication
Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays, Hui-Yu Liu, Ramsha Masood, & Michael Hirtz et al., Advance Materials, 35, 33, 2021, https://doi.org/10.1002/adma.202008493
Timeline
Scientific researcher
Laboratory for Translational Research at Department for Internal Med 1. University Medical Center Hamburg Eppendorf
04.2023 - Current
Biologist
Exosomes and Tumor biology laboratory, Institute for Infection Prevention and Hospital Epidemiology, Universität Klinikum Freiburg Germany
04.2021 - 02.2023
Research Assistant
Exosomes and Tumor biology laboratory, Institute for Infection Prevention and Hospital Epidemiology, Universität Klinikum FreiburgGermany
03.2020 - 03.2021
Diploma of Advanced Studies in Biomedical Science - Biomedical Sciences
Albert-Ludwigs University of Freiburg
10.2018 - 02.2019
International Double Master’s in Biomedical Science (M.Sc.) - Biomedical Sciences
Albert-Ludwigs University of Freiburg, Germany And University of Buenos Aires, Argentina
02.2018 - 02.2020
Biologist
Dr. Shamsi & Ansari Diagnostics
05.2012 - 05.2013
Bachelors in Biomedical sciences - Biomedical Sciences
Dow Medical University
01.2009 - 07.2013
High School - Biology
St. Joseph’s College For Women
09.2006 - 09.2008
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